![phylogenetics - Why do NEBNext indexing primers have sequence between the p5 oligo and index? - Bioinformatics Stack Exchange phylogenetics - Why do NEBNext indexing primers have sequence between the p5 oligo and index? - Bioinformatics Stack Exchange](https://i.stack.imgur.com/7Gc73.png)
phylogenetics - Why do NEBNext indexing primers have sequence between the p5 oligo and index? - Bioinformatics Stack Exchange
TraDIS Supplement 1 Contents: TraDIS adapter and primer design: pages 2 - 4 MiSeq and HiSeq recipe description: pages 5 - 8
P5: 5' AAT GAT ACG GCG ACC ACC GA 3' P7: 5' CAA GCA GAA GAC GGC ATA CGA GAT 3' When checking your libraries by cloning and stand
![Adapterama I: universal stubs and primers for 384 unique dual-indexed or 147,456 combinatorially-indexed Illumina libraries (iTru & iNext) [PeerJ] Adapterama I: universal stubs and primers for 384 unique dual-indexed or 147,456 combinatorially-indexed Illumina libraries (iTru & iNext) [PeerJ]](https://dfzljdn9uc3pi.cloudfront.net/2019/7755/1/fig-4-2x.jpg)
Adapterama I: universal stubs and primers for 384 unique dual-indexed or 147,456 combinatorially-indexed Illumina libraries (iTru & iNext) [PeerJ]
![phylogenetics - Why do NEBNext indexing primers have sequence between the p5 oligo and index? - Bioinformatics Stack Exchange phylogenetics - Why do NEBNext indexing primers have sequence between the p5 oligo and index? - Bioinformatics Stack Exchange](https://i.stack.imgur.com/LHZV0.jpg)
phylogenetics - Why do NEBNext indexing primers have sequence between the p5 oligo and index? - Bioinformatics Stack Exchange
![A) Regular Illumina multiplex library design. The grafting sequences... | Download Scientific Diagram A) Regular Illumina multiplex library design. The grafting sequences... | Download Scientific Diagram](https://www.researchgate.net/publication/51736748/figure/fig2/AS:324862276063271@1454464610805/A-Regular-Illumina-multiplex-library-design-The-grafting-sequences-P5-and-P7-are.png)
A) Regular Illumina multiplex library design. The grafting sequences... | Download Scientific Diagram
![Primer construct and amplification products. The primers are composed... | Download Scientific Diagram Primer construct and amplification products. The primers are composed... | Download Scientific Diagram](https://www.researchgate.net/publication/283456688/figure/fig2/AS:613934851686442@1523384883596/Primer-construct-and-amplification-products-The-primers-are-composed-of-sequences.png)