![SOLVED: You are working on gradient PCR to determine the Optimum annealing temperature for your primer; and you get the following resull; SOLVED: You are working on gradient PCR to determine the Optimum annealing temperature for your primer; and you get the following resull;](https://cdn.numerade.com/ask_images/0e8cd6a302ba402daf7bb41a57e29315.jpg)
SOLVED: You are working on gradient PCR to determine the Optimum annealing temperature for your primer; and you get the following resull;
![SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (% SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%](https://cdn.numerade.com/ask_previews/6f0c47ee-38d7-4778-b4d8-d117b7d1affe_large.jpg)
SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
![Detecting eukaryotic microbiota with single-cell sensitivity in human tissue | Microbiome | Full Text Detecting eukaryotic microbiota with single-cell sensitivity in human tissue | Microbiome | Full Text](https://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs40168-018-0529-x/MediaObjects/40168_2018_529_Fig1_HTML.png)
Detecting eukaryotic microbiota with single-cell sensitivity in human tissue | Microbiome | Full Text
Skaistė Valinskytė, Edita Elijošiūtė, Tomas Radzvilavičius, Miglė Mikutėnaitė, Jonas Belevičius, Aistė Serapinaitė,
![Optimization of Annealing Temperature To Reduce Bias Caused by a Primer Mismatch in Multitemplate PCR | Applied and Environmental Microbiology Optimization of Annealing Temperature To Reduce Bias Caused by a Primer Mismatch in Multitemplate PCR | Applied and Environmental Microbiology](https://journals.asm.org/cms/10.1128/AEM.67.8.3753-3755.2001/asset/7d57d42d-fcda-4bdf-94fb-bfbf43965510/assets/graphic/am0810201001.jpeg)
Optimization of Annealing Temperature To Reduce Bias Caused by a Primer Mismatch in Multitemplate PCR | Applied and Environmental Microbiology
![Figure 1 from Controlled hot start and improved specificity in carrying out PCR utilizing touch-up and loop incorporated primers (TULIPS). | Semantic Scholar Figure 1 from Controlled hot start and improved specificity in carrying out PCR utilizing touch-up and loop incorporated primers (TULIPS). | Semantic Scholar](https://d3i71xaburhd42.cloudfront.net/99913474d2cc168c4f3ea76004c7fafd02fbbef6/1-Figure1-1.png)